Frequently Asked Questions
Question: How do I set up a billing code to reserve instrument time?
Answer: Review our billing policies and submit a request for billing code here.
You may also contact Angie Fuhr- firstname.lastname@example.org – AMRIS Office Manager.
Question: What is your mailing address?
University of Florida
P.O. Box 100015
Gainesville, FL 32610-0015
Question: What is your shipping address?
University of Florida
1149 Newell Drive, RM LG-117
Gainesville, FL 32611
Question: How do I transfer my data from the facility after collection?
Answer: The answer depends on which system the data was collected from.
For the 500, 600, Cryo600 and the 750 instruments, the data should be transferred to the datastation after collection via the cross-mounted network. From the data station the data may be transferred via ftp using filezilla.
For the horizontal 4.7T and the 11T systems, you may ftp directly from the system.
Question: How do I apply for NHFML user programs?
Answer: Please submit a proposal through the user portal (http://users.magnet.fsu.edu ) and select the AMRIS facility. The proposal will be peered-reviewed by members of the NHMFL user committee. You will receive reviews of your proposal in 2-3 weeks.
Question: How do I Remotely Operate an Instrument?
Answer: To gain access to the AMRIS facility systems remotely, you will need to have a UFID and gatorlink account. If you don’t have a UFID, contact Angie Fuhr. Once a UFID is issued, she will send it to you so that you can create a gatorlink account. The next is to go to https://my.ufl.edu/psp/ps_pwd/EMPLOYEE/EMPL/c/UF_PA_GL_ACCT_MGMT.UF_PA_SS_GL_CREATE.GBL and enter the information needed to create your gatorlink account, which includes your UFID number. Once you have a gatorlink account, you need to set up VPN access. Directions are at https://connect.ufl.edu/it/wiki/Pages/glvpn.aspx
The AMRIS staff person responsible for the instrument you will be working on will set up your instrument login and send the address for the machine you wish to use, your username and password.
IMPORTANT: you should only login to the console software when you have time booked on the machine. Logging in at other times may impact other users. Remote running of instruments is strictly restricted to experienced users.
Question: What if a study participant has worked with metal or been injured by a foreign object and needs an orbital X-ray to be cleared for entering the 3T MRI magnet?
Question: What are the technical capacities of the 3T Facility?
Answer: Structural and functional MRI
Methods and protocols for MR data acquisition
Image quality assurance and quality control
Image acquisition and transfer support
Image archive, review, and access
Teaching investigators data acquisition and analysis techniques
Assisting researchers in designing experimental protocols
Development of advanced MR imaging and spectroscopy methodology
Question: What technical support is provided for the use of the 3T facility?
Answer: A research-dedicated technologist is available to operate the scanner and fMRI paradigms 8-2:30pm, Monday-Friday. Training is provided to researchers to run the scanner if their projects are funded for multiple years and late afternoon or evening or weekend scanning is involved. We provide support in imaging protocol development, including pulse sequence, fMRI paradigm, as well as image processing and analysis. Depending on the scope of our involvement, this typically can be arranged in form of research collaboration. We provide support for integrating peripherals researchers bring in for their projects with the scanner and ESys®.
Question: How do I make arrangements to use AMRIS’s Agilent/Varian VNMRS-600 spectrometer?
Answer: The spectrometer is located in Room 100 of the Chemistry Laboratory Building (CLB) on the main UF campus, about a 3/4-mile walk north from the AMRIS Facility of the McKnight Brain Institute (MBI) in the UF Health Science Center. One can type “CLB” and “MBI” into the “Search” window on the following web page in order to see the locations of these buildings on a map of the University of Florida.
Contact Jim Rocca by e-mail (email@example.com) or phone (352-294-0126) concerning this instrument. The spectrometer is operating with Agilent’s VNMR-J software version 3.2, the same software which is running on the Chemistry Department’s Varian 500 MHz spectrometers and two of its Varian 300 MHz spectrometers. If you are already familiar with that software, then Mr. Rocca can provide a check-out for you on the VNMRS-600 concerning to the particulars of its operation. Once you are comfortable operating the instrument on your own, you can then make arrangements for independent key access to CLB-100, as well as for building access if you are not a member of the Chemistry Department. Please note that such keys are not issued to individuals, but to a single research group, and the Professor overseeing your research will need to sign the form requesting access to this spectrometer.
Question: I only have a small amount of a substance, e.g., natural product, poly-peptide, etc. What AMRIS spectrometer(s) should I use to obtain the best spectra on my sample in solution, and is it possible to have assistance with the spectrometers?
Answer: Knowing some additional details about the “small” sample will help decide which instrument to use: if known, what its approximate molecular mass is, what types of NMR spectra you require, and whether you have micro-grams or milli-grams of the substance. For further information about how AMRIS’s liquid-state spectrometers may meet your mini-/micro-sample needs, please contact Jim Rocca by e-mail (firstname.lastname@example.org) or phone (352-294-0126). Generally speaking, our 600-MHz instruments equipped with cryogenically-cooled radio-frequency (RF) probes will provide you with the best sensitivity for proton(1H)-detected spectra. Both spectrometers have commercial 5-mm indirect-detection cryogenic probes. Additionally, there are occasionally available unique, prototype high-temperature-superconducting (HTS) cryogenic “RF micro-probes” that may be more suitable for your sample.
• The 1-mm HTS probe for the BrukerAvance-II-600
• The 1.5-mm HTS probe for the Agilent VNMRS-600
If you also hope to obtain directly-detected carbon (13C) spectra on your small sample, then the Agilent system, with either its 5-mm or 1.5-mm cryogenic RF probe, will provide the best sensitivity.
If you are already familiar with Bruker’s TopSpin software or Agilent’s VNMR-J software, then Mr. Rocca can provide a check-out for you on either spectrometer. If you require training for solution NMR on any of these systems, or if you would prefer to simply have an operator provided for your sample analysis on one of the spectrometers, then Jim is also the AMRIS staff member to contact.